PCR workstation Hood

Purifier Filtered PCR Enclosures

Purifier Filtered PCR Enclosures are vertical clean benches that protect your work. Available in 2', 3' and 4' widths, they provide a controlled environment in which to perform polymerase chain reaction experiments. ISO Class 5 air is constantly projected vertically down over the work area, minimizing the risk of cross contamination of samples.

Purifier Filtered PCR Enclosures do not provide protection to the user and should not be used in conjunction with biohazardous material, toxins or radionuclides. You and your safety officer must carefully assess risks associated with any operation performed in the PCR enclosure.

UV Decontamination Timer

The variable digital timer provides UV light exposure continuously or in user-selected increments of 5, 10, 15, 30, 60, 120 or 240 minutes to deactivate DNA and RNA contaminants. When the timer expires, the UV light automatically switches off in preparation for the next experiment. An interlocked protection panel guards against inadvertent exposure to UV rays.

Airflow Operation

During operation, room air is drawn through a prefilter in the top of the cabinet to trap large particles. Then, the air flows through a 99.99% efficient HEPA filter and is projected vertically over the work area. This HEPA-filtered air prevents contaminants from entering the work area and provides a particulate-free work environment that minimizes cross contamination.

Features and Benefits

• Particulate-free ISO Class 5 air: Particle counts in the work area conform to ISO 14644-1 and 2, providing ISO Class 5 conditions (formerly Class 100 per Federal Standard 209E), ensuring fewer than 3520 particles 0.5 μm or larger per cubic meter of air
• HEPA filter: Rated to remove 99.99% of all particles 0.3 micron in size; industry standard size for economical replacement
• Disposable prefilter: Traps large particles to extend the life of the HEPA filter
• Tempered safety glass sash: Sloped 10° for less glare and closer, more comfortable viewing than vertical sashes offer; UV opaque and resistant to UV irradiation; wiping seals ensure ISO Class 5 conditions inside the enclosure; spring-loaded latch allows sash to be pivoted upward and locked open for loading and cleaning
• Variable digital timer: Provides timed UV light exposure from front-mounted control panel; operator may select from eight settings in minutes of 5, 10, 15, 30, 60, 120, 240 and continuous; amber LED indicator blinks for the duration of the setting
• Ultraviolet lamp (254 nm): Interlocked with protection panel, denatures stray DNA strands and provides secondary decontamination while the enclosure is not in use; two top-mounted support hooks allow the protection panel to be stowed above the work area while the bench is in use
• Protection panel: Interlocked with UV lamp, closes off the sash opening to guard against inadvertent UV light exposure and prevent contaminants from entering when the enclosure is not in use
• Variable speed blower: Solid state control may be accessed by a certified technician to validate and adjust downflow velocity
• Light and blower switches: Located within easy reach of the operator; 3-way light switch powers the fluorescent and UV light, but only one at a time, helping to protect the operator from inadvertent UV exposure
• Front-mounted differential pressure gauge: Indicates the total system pressure across the HEPA filter and pressurized plenum to help determine HEPA filter loading
• Air diffuser: Protects the HEPA filter from possible damage from direct contact with the operator and apparatus inside the enclosure; distributes air evenly across the work area to optimize airflow
• Glare-free fluorescent lighting: Lamp is located above the work area, out of contact with the filtered air
• Two utility ports: Iris openings allow passage of tubing and electrical cords from equipment inside the enclosure through the back for connection to services; plugs are provided to seal the ports when not in use
• ETL listed: All 115 volt, 60 Hz models carry the ETL mark signifying that they are certified to UL Standard 3101-1/61010-1 and CAN/CSA C22.2 No. 1010.1
• CE mark: All 230 volt, 50 Hz models conform to the CE (European Community) requirements for electrical safety and electromagnetic compatibility
• Optional Guardian Airflow Monitor: Alerts the user to downflow velocities outside of preset range and HEPA filter conditions

Standard Features

• Nominal downflow velocity of 40-55 fpm
• Supply HEPA filter, 99.99% efficient on particles 0.3 micron
• ISO Class 5 air (formerly Class 100 per Federal Standard 209E)
• Disposable prefilter
• Variable speed built-in blower with motorized impeller and solid state control rated for 5 amps
• Differential pressure gauge
• Air diffuser
• UV-resistant, glacier white and gray powder-coated aluminum and steel construction
• UV-opaque and resistant 1/4" thick tempered safety glass sash and sides
• 9.4" high sash opening
• Fluorescent lamp
• 254 nm UV lamp
• Variable digital timer for UV exposure with eight settings in minutes of 5, 10, 15, 30, 60, 120, 240 and continuous
• Blower and 3-way light (fluorescent, UV and off) switches
• Ergonomic 10° angled and hinged sash that pivots upward, locks to a loading height of 20" and has wiping seals to promote ISO Class 5 conditions
• Protection panel interlocked with UV lamp that closes the sash opening and two support hooks located atop the enclosure for stowing the panel
• Two utility ports with iris openings and plugs
• ETL listing, UL 3101-1/61010-1 and CAN/CSA C22.2 No. 1010.1 conformity (115 volt models)
• CE Conformity Marking (230 volt models)
• One year warranty on parts and labor

Standard Option

Guardian Airflow Monitor: Continuously monitors downflow airflow and has two LED lights to indicate when airflow is within or outside set point range. Set point is factory calibrated for 25-35 fpm downflow velocity. The "Safe" green LED glows when airflow is within set point range. The "Alert" red LED glows and an audible alarm sounds when airflow is outside set point range. The audible alarm may be muted by pressing the "Silence Alarm" switch.

Model Specifications

*International electrical configuration

Dimensions

Additional Dimensions (All Models)

• Exterior Height: 36.8" (93.5 cm)
• Exterior Depth: 28.6" (72.6 cm)
• Interior Height: 22.7" (57.7 cm)
• Interior Depth: 25.3" (64.3 cm)
• Sash Height: 9.4" (23.9 cm)
• Visibility: 20.8" (52.8 cm)
• Controls: 24.9" (62.2 cm)

Required Accessories

• Supporting work surface. Place on existing casework or see Solid Epoxy Dished Work Surfaces below.
• Supporting base. Place on existing casework or see Base Stands below.

Accessories

Solid Epoxy Dished Work Surfaces

Black chemical-resistant work surface is contoured to fit the dimensions of the Purifier Vertical Clean Benches and Filtered PCR Enclosures and to contain spills.