PCR Workstation

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PCR Laminar Flow Cabinets

The Purair PCR laminar flow cabinet employs Air Science Multiplex HEPA Filtration technology to create a safe, energy-efficient, contaminant-free environment. Designed for PCR applications, these cabinets provide a sterile work zone with vertical laminar airflow while protecting against cross-contamination. Available in three widths—24", 36", and 48"—the cabinets feature 360 degree visibility with UV absorbing polycarbonate construction and are ideally suited for use when flexible access to instrumentation inside the cabinet is required.

PCR Workstation Overview

Polymerase chain reaction (PCR) is a simple and inexpensive technique to make multiple copies of a targeted nucleotide sequence from a DNA sample and to amplify sequences from small samples. This technique is widely used in genetics laboratories that work with DNA and RNA. Because PCR amplification is extremely sensitive to contamination, prevention of contamination requires good laboratory practices to minimize external or cross-contamination during reagent preparation, sample preparation and sample amplification.

Intended Applications

PCR cabinets are intended for use in non-hazardous applications where biological or biohazard byproducts are not generated and user protection is not required.

• PCR Laboratories
• Forensics
• Pharmaceutical
• Sample Preparation
• General Research Protocols

Energy Efficiency

The single EC blower motor assures lower cost of ownership in one of the world's most energy efficient laminar flow cabinets, consuming only 37–115 watt depending on cabinet size. Energy-efficient centrifugal blowers promote long life and dependable performance of Purair PCR work stations.

Air Cleanliness and Flow

Air cleanliness meets ISO Class 5. PCR work stations maintain an average airflow velocity of 0.30 m/s (60 fpm) at initial setpoint, measured 6" (150 mm) from the filter face. Room air enters from the top of the cabinet through the disposable pre-filter where larger particles are trapped, increasing the service life of the main HEPA filter.

Air is forced across the HEPA filter to deliver a flow of pure air within the work zone to dilute and flush airborne contaminants from the work area. An average airflow velocity of 0.30 m/s (60 fpm) ensures that there is sufficient number of air changes within the work zone to eliminate cross-contamination and to maintain optimum cleanliness. Purified air travels across the work zone to the work surface in a vertical downflow stream and then exits the work zone across the open cabinet front.

Construction and Materials

• White epoxy-coated steel frame with white legs on cabinet sides
• Polycarbonate, transparent, UV absorbing windows
• Standard black polypropylene work surface; optional white polypropylene or stainless steel
• Large polypropylene work surface with optional stainless steel work surface available
• Clear back and side panels allow ambient light into the chamber and provide users with an unobstructed view of contents
• Stackable sections make cabinets highly portable and enable quick setup

Filtration System

At the heart of the PCR work station is innovative filtration technology. The Multiplex Filtration System consists of a pre-filter and main filter. The mechanical design enhances safety, convenience and overall value.

• The disposable pre-filter is accessible from the exterior top of the cabinet
• The pre-filter may be replaced while unit is in operation
• A filter clamping mechanism allows for the filter to be easily installed and ensures an even seal at the filter peripheral face at all times to prevent bypass leakage
• Screw compression clamp
• The filter chamber prevents contaminated air from contacting internal cabinet mechanisms
• The HEPA filters are easy to replace; no tools required
• The main filter is easy to replace; no tools required. The filter clamps tightly against the filter gasket to prevent filter bypass and to maintain filter integrity

Air Science uses long-life HEPA filters without aluminum separators to increase filter efficiency, minimize the potential for leakage and increase filter life. The Air Science filter assembly is easy to access, easy to change, plus a unique filter clamping design eliminates bypass leakage of the main filter.

Decontamination Features

• Equipped with germicidal UV lamp to create light emission conditions known to provide safe decontamination
• Built-in ultraviolet lamp with timer creates light emission conditions known to permit safe decontamination between PCR cycles
• 1 × 253.7 nanometer 15 watt UV lamp
• Includes a double-flap safety cover of 0.2" (5 mm) UV absorbing 254 nm beta radiation resistant polycarbonate; provides superior operator protection while allowing easy access to the work zone
• Timer can be set over range of 0 to 60 minutes or set to HOLD
• Decontamination shelf mounted on the back wall near the UV lamp for maximum exposure
• Magnetic door latch with safety interlock prevents operator exposure to UVs when door is opened during decontamination cycle

Control and Safety

• Integrated control panel features an On/Off switch for the fan, light and UV lamp timer
• Main On/Off switch for fan and lighting
• Solid-state fan speed control with RFI filter maintains blower uniformity
• UV timer, safety interlock shut-off
• Filter door lock prevents unauthorized removal or accidental exposure to dirty filters
• Double-hinged self-locking front sash with magnetic sash closure protects samples on the work surface from contamination with up to 90 fpm airflow

Ergonomic and Functional Design

• Ergonomically angled front improves reach and user comfort
• Energy saving LED lighting
• Compact LED cabinet lamp located away from laminar flow area
• Convenient rear wall pass-through ports for safe routing of instrument cords, cables and leads
• Two standard, knockout pass through ports
• Optional base stand converts to mobile cart with optional locking casters
• An optional stainless steel work surface includes lips on all four sides to contain spills

Available Models

Purair PCR cabinets are available in three model sizes with various options. Designed for desktop use or installation on an optional base stand or mobile cart. All models are available in 120V, 60Hz or 230V, 50Hz models.

Power Consumption by Model

Options and Accessories

Standards and Compliance