PCR Workstation

PCR Laminar Flow Cabinets

The Purair PCR laminar flow cabinet employs Air Science Multiplex HEPA Filtration technology to create a safe, energy-efficient, contaminant-free environment. These professional-grade work stations provide sterile work zones for aseptic techniques, making them ideally suited for applications requiring flexible access to instrumentation inside the cabinet with 360 degree visibility.

Applications

PCR cabinets are intended for use in non-hazardous applications where biological or biohazard byproducts are not generated and user protection is not required.

• PCR Laboratories
• Forensics
• Pharmaceutical
• Sample Preparation
• General Research Protocols

PCR Workstation Overview

Polymerase chain reaction (PCR) is a simple and inexpensive technique to make multiple copies of a targeted nucleotide sequence from a DNA sample and to amplify sequences from small samples. This technique is widely used in genetics laboratories that work with DNA and RNA. Because PCR amplification is extremely sensitive to contamination, prevention of contamination requires good laboratory practices to minimize external or cross-contamination during reagent preparation, sample preparation and sample amplification.

Performance Characteristics

PCR work stations maintain an average airflow velocity of 0.30 m/s (60 fpm) at initial setpoint, measured 6" (150 mm) from the filter face. The Air Science Multiplex Filtration System offers a range of options for high performance protection. Multiplex filter configuration permits a customized combination of filter media for a broad range of chemical families and biological agents if required.

Air cleanliness meets ISO Class 5. Energy-efficient centrifugal blowers promote long life and dependable performance. All models feature energy consumption ranging from 37 to 115 watts, making these among the world's most energy efficient laminar flow cabinets.

Key Design Elements

• Vertical laminar airflow with HEPA filtration
• 360 degree visibility with UV absorbing polycarbonate construction
• Equipped with germicidal UV lamp to create light emission conditions known to provide safe decontamination
• Energy saving LED lighting
• Stackable sections make cabinets highly portable and enable quick setup
• Professional quality design complies with current technical and safety regulations
• Available in three model sizes with various options
• Designed for desktop use or installation on optional base stand or mobile cart

Airflow and Filtration System

Airflow Pattern

Room air enters from the top of the cabinet through the disposable pre-filter where larger particles are trapped, increasing the service life of the main HEPA filter. Air is forced across the HEPA filter to deliver a flow of pure air within the work zone to dilute and flush airborne contaminants from the work area. An average airflow velocity of 0.30 m/s (60 fpm) ensures that there is sufficient number of air changes within the work zone to eliminate cross-contamination and to maintain optimum cleanliness. Purified air travels across the work zone to the work surface in a vertical downflow stream and then exits the work zone across the open cabinet front.

Multiplex Filtration System

At the heart of the PCR work station is innovative filtration technology. The Multiplex Filtration System consists of a pre-filter and main filter. The mechanical design enhances safety, convenience and overall value.

• The disposable pre-filter is accessible from the exterior top of the cabinet
• A filter clamping mechanism allows for the filter to be easily installed and ensures an even seal at the filter peripheral face at all times to prevent bypass leakage
• The filter chamber prevents contaminated air from contacting internal cabinet mechanisms
• The pre-filter may be replaced while unit is in operation
• The main filter is easy to replace; no tools required

The Air Science filter assembly is easy to access and easy to change, plus a unique filter clamping design eliminates bypass leakage of the main filter. Air Science uses long-life HEPA filters without aluminum separators to increase filter efficiency, minimize the potential for leakage and increase filter life.

Construction and Components

Structural Features

• Control Panel: The integrated control panel features an On/Off switch for the fan, light and UV lamp timer
• Main Filter: Main HEPA filter with 99.97% efficiency for 0.3 micron particulates (ULPA optional)
• Pre-Filter: The pre-filter can be changed while the unit is operating to prevent operator exposure to chemical vapors
• Fan: High-performance EC fan
• Stand: Optional base stand converts to mobile cart with optional locking casters
• Lighting: Compact LED cabinet lamp located away from laminar flow area
• UV Lamp: Built-in ultraviolet lamp with timer creates light emission conditions known to permit safe decontamination between PCR cycles. Includes a double-flap safety cover of 0.2" (5 mm) UV absorbing 254 nm beta radiation resistant polycarbonate; provides superior operator protection while allowing easy access to the work zone. Timer can be set over range of 0 to 60 minutes or set to HOLD
• Pass Through Ports: Convenient rear wall pass-through ports for safe routing of instrument cords, cables and leads
• Filter Door Lock: Prevents unauthorized removal or accidental exposure to dirty filters
• Ergonomic Design: Ergonomically angled front improves reach and user comfort
• Work Surface: Large polypropylene work surface, white or black standard with optional stainless steel work surface available
• Decontamination Shelf: Mounted on the back wall near the UV lamp for maximum exposure
• Magnetic Door Latch: Safety interlock prevents operator exposure to UVs when door is opened during decontamination cycle
• Double-Hinged Self-Locking Front Sash: The magnetic sash closure protects samples on the work surface from contamination with up to 90 fpm airflow

Additional Design Benefits

Clear back and side panels allow ambient light into the chamber and provide users with an unobstructed view of contents. The cabinet frame and work surfaces, comprised of industrial components, are durable and chemically resistant. An optional stainless steel work surface includes lips on all four sides to contain spills. All models are available in 120V, 60Hz or 230V, 50Hz models.

Technical Specifications

Model Dimensions and Weight

Product Specifications

Options and Accessories