Purair BIO Class II, Type A2 Biological Safety Cabinet
The Purair BIO biological safety cabinet delivers exceptional energy efficiency and reliable performance for life science applications. Featuring a single EC brushless DC motor design, these cabinets consume just 220–490 watts while providing HEPA-filtered protection for operators, products, and the environment. Available in four sizes, the Purair BIO offers NSF 49 and EN12469 certified containment suitable for biosafety levels 1, 2, and 3.
Model AS-AHA-133-CA-B
This 4-foot model features standard membrane-style controls, 115V AC 60Hz operation, and EN12469 certification. The cabinet provides 99.99% particulate filtration efficiency at 0.3 microns through dual HEPA filters while maintaining energy consumption at just 300 watts.
Energy Efficiency and Motor Design
The Purair BIO utilizes a single EC brushless DC motor that eliminates the complexity and expense of dual motor systems. This design ensures balanced airflow, simplified maintenance, and lower operational costs while converting over 80% of motor output power to kinetic energy. The motor automatically adjusts speed to maintain compliant airflow while compensating for filter loading and voltage fluctuations.
Applications
- Life Science Research
- Sterile Product Preparation
- Biological Protocols
Containment and Airflow Performance
The cabinet maintains a 70% recirculation to 30% exhaust airflow ratio, with average inflow velocity of 100 fpm (0.51 m/s) and downflow velocity of 60 fpm (0.30 m/s). Negative pressure inside the cabinet during operation prevents contaminants from escaping the work area. The integrated HEPA filtration system delivers clean air in a gentle vertical laminar flow pattern while the exhaust HEPA filter traps biohazardous particles before air returns to the room.
Protection Features
- Dual HEPA filtration: supply and exhaust filters rated 99.99% efficient at 0.3 microns (Class H14)
- Double-wall plenum design surrounds contaminated areas with negative pressure
- Surround air intake grille contains all contaminated air within the work zone
- 6 mm anti-ultraviolet tempered glass window for operator protection
- 10° angled window design reduces operator fatigue and eye strain
- Safety interlock feature automatically controls fan, lighting, and UV lamp based on sash position
Operational Features
- Analog Minihelic gauge verifies airflow
- On/Off key-switch controls main power
- Membrane-style soft switches control interior lighting, UV lamp, outlet activation, and fan
- Sash alarm with audible and visual warning at unsafe position (10 inches or 25 cm from cabinet bottom)
- Mute button silences alarm for specified time
- Patented Quick Access HEPA filtration lock allows front-side filter changes by single person without tools
- Two 70 mm NSF-approved pass-thru ports on interior sidewalls for cord passage
- Paper catch screen at rear air return plenum
Construction and Ergonomics
The cabinet features SUS#304 stainless steel construction with a heavy-gauge, one-piece liner and coved corners for durability. The stainless steel elbow/arm rest provides ergonomic forearm support to prevent grille blockages and improve posture. Key components including fluorescent lamps, motor capacitor, electrical harness, electronic ballast, and switch control are mounted outside the airstream for service without decontamination.
Electrical and Service Connections
- Two duplex electrical outlets with splash-proof, UL listed covers
- Optional CSA certified service valves for gas, air, and vacuum (maximum working pressure 75 PSI)
- 115V, 60Hz, 15A electrical requirement
Installation Advantages
The slim profile design (31.1" deep) passes easily through standard 38" wide doorways. Self-contained operation requires no venting to the outside, eliminating facility ductwork costs and reducing HVAC make-up air requirements. Units are portable and may be moved between locations with minimal downtime and without filter changes.
Technical Specifications
| Specification |
Value |
| Model |
AS-AHA-133-CA-B |
| Voltage |
115V, AC, 60Hz |
| Certifications |
EN12469, IEC61010-1 |
| NSF 49 Certified |
Yes |
| Internal Dimensions (W × D × H) |
48" × 24.6" × 28" (1220 × 626 × 709 mm) |
| External Dimensions (W × D × H) |
52.5" × 31.1" × 60.5" (1334 × 789 × 1537 mm) |
| Shipping Dimensions (W × D × H) |
55.1" × 33.5" × 77.8" (1400 × 850 × 1950 mm) |
| Net Weight |
529 lbs / 240 kg |
| Shipping Weight |
617 lbs / 280 kg |
| Power Consumption |
300 watt |
| Window Operation |
8" / 20 cm |
| Room Height Required |
89.4" / 227 cm |
Performance Specifications
| Parameter |
Specification |
| Airflow Ratio |
70% recirculated / 30% exhausted |
| Average Inflow Velocity |
100 fpm (0.51 m/s) |
| Average Downflow Velocity |
60 fpm (0.30 m/s) |
| Exhaust Air Volume with Exhaust Canopy |
459 cfm (13 m³) |
| Supply HEPA Filter |
(1) 99.99% @0.3 microns (Class H14) |
| Exhaust HEPA Filter |
(1) 99.99% @0.3 microns (Class H14) |
| Construction |
SUS#304; 6 mm tempered window glass |
| Blower |
EC brushless DC Motor |
| Electrical Switches |
Membrane push switches; On/Off |
| Electrical Outlets |
(2) Duplex electrical outlets with splash-proof cover (UL listed) |
| Fluorescent Intensity |
> 1,000 lux |
| Fluorescent Lamp |
(2) T5, 28 watt |
| UV Lamp |
(1) FL 30 watt, 254 nm |
| Noise Level |
< 63 dBA |
Available Options and Accessories
| Option |
Description |
Part Number |
| Exhaust Collar |
For connecting the biosafety cabinet to building exhaust or remote blower for outside ducting |
HBE-133-AA-B |
| Service Fixture Kit |
Additional connections for Air/Gas/Vacuum. CSA certified |
BA-L4100-158-B |
| IV Bar Kit |
Includes 6 hooks. Specify when ordering. Field installed |
BC-1671-A |
| Moveable Elbow / Arm Rest |
Made of stainless steel #304 and provides ergonomic forearm support to prevent grill blockages and improve posture |
SHA-1X3-AA-A |
| Microprocessor Control |
Full-color microcomputer touch panel with graphical user interface. Real time digital display of inflow velocity, downflow velocity, HEPA filter pressure and usage time for HEPA filters and UV lamps |
AS-AHA-133-AA-B or AS-AHA-133-AB-B |
Standards and Compliance
| Standard |
Certification |
| Quality Management Systems |
ISO 9001:2015, ISO 14001:2015 |
| Cabinet Performance |
EN12469, NSF 49 |
| Electrical Safety |
UL-C-61010-1, CAN/CSA C22.2 61010-1-12, EN 61010-1:2010 |
| CE Mark |
Yes |
NSF Certification applies to biological safety cabinet models AHA-133-AA-B (Air Science Model AS-AHA-133-AA-B), AHA-133-AB-B (Air Science Model AS-AHA-133-AB-B), AHA-133-CA-B (Air Science Model AS-AHA-133-CA-B), AHA-133-CB-B (Air Science Model AS-AHA-133-CB-B), AHA-193-AA-A (Air Science Model AS-AHA-193-AA-A), AHA-193-AB-A (Air Science Model AS-AHA-193-AB-A), AHA-193-CA-A (Air Science Model AS-AHA-193-CA-A), AHA-193-CB-A (Air Science Model AS-AHA-193-CB-A) manufactured by Chung Fu Technical Development Co. Ltd., Taipei, Taiwan and marketed by Air Science, LLC, USA.
